Enterococcus faecalis gram stain pictures


  • Gram positive bacteria – Enterococcus
  • Streptococcus
  • The study was conducted on 2 isolates from the blood samples to become the number of isolates 36 isolates. And the study showed that type E. K , Hussein J. M, Kadhem E. J, Al-Hadad A. Biomed Pharmacol J ;13 2. These bacteria can cause a wide range of diseases if they affect the urinary tract. Gilmore et al. Nowadays, enterococci have become the second most common bacterial cause of acquired infections, causing many therapeutic difficulties, also thier resistance to new antibiotics due to widespread use of these antibiotics leading to true resistance by enterococci.

    Several experiments on laboratory animals have demonstrated the ability of these cocci to cause many infections as they have multiple virulence factors, which can be divided into secretory virulence factors such as Haemolysin, Protease, Hyaluronidase, Bacteriocin, Pheromones and Lipase, as well as aggregate proteins Agents. Polysaccharides wall cell, and some studies have shown that they have the ability to produce a capsule Sava et al.

    As a pathogen acquired community, the bacterium enterococci became involved in increasing the number of poultry deaths alchowdhury, , Leavis at el. Despite the medical development in the field of microorganisms, the incidence of opportunistic pathogens and acquired infections, including enterococcus bacteria, is still causing many medical problems. Thus the goal of this study Conducting a local study of these bacteria in cattle females. Isolation and diagnosis of enterococci from different pathogenic models in cows and their diagnosis at the species level.

    Studying virulence factors which include: Study of characteristic enzymes. Investigating the existence of the capsule Study of the ability of bacteria to cause blood clotting and the effect of mannose sugar in the process of clotting. For 15 minutes Collee et al.

    The medium was used to detect the ability of gastrointestinal for motility Langston. Bile salt esculin agar, which has been synthesized from its base materials according to Finegold and Baron, Potassium Tellurate Salinity Tolerance Test 0. The three-axis modification medium. This medium was used for the rapid initial diagnosis of enterococci bacteria and includes three biochemical tests in one medium: a bacterial isolation test for hydrolyzed insulin, a salinity tolerance test, a motion test, and according to the basic components as described in HannaNo, Cultural, microbiological and biochemical characteristics were determined.

    Detection of Virulence Factors Possessed by Bacterial Isolates Investigation Lipase Production: This medium used to investigate Lipase production was used for this purpose as it was prepared from its basic materials as stated in Starr, The appearance of a transparent halo around the colonies is an indication of positive screening Screening for Protease Production: Three methods were used to prepare the screening medium for protease production:- Trypticase soya agar medium was prepared according to the instructions of the company Himedia and added 1.

    The appearance of a transparent halo around the growing colonies in the prepared medium indicates a positive test. The coloration of the capillary tubes in yellow indicates positive test Elsner et. Capsule Production Test The Indian ink pigmentation method was used to detect the presence of the capsule A portion of the colony was taken and placed on the glass slide and a drop of Indian ink was added to it and blended, Then spread the mixture on the surface of the slide and left to dry and examined by microscope Bottone al et.

    Results and Discussion Isolation and Diagnosis The number of isolates obtained from a total of different pathological samples reached 34 isolates, distributed between 21 And the results of the microscopic examination, the colonies of the bacteria showed in the form of single and double cocci, some of which were in the form of short chains positive for Gram-stain dye 2.

    Biochemical Tests The results showed that all strains were negative for the tests of catase ,the production of dyes and movement and positive tests for salinity and base tolerance and the hydrolyse of ascolin and the tolerance of bile salts, in addition to its ability to tolerate 0.

    The results also showed that the strains of type E. While E. Table 1: Shows the biochmical diagnostic tests for enterococcus bacteria. Initial tests.

    Viridans streptococci S. Represents different groups of streptococci, including the S. Nonencapsulated S. Capsular production is lost by all encapsulated pneumococci after passing through a few subcultures on agar, but they will again produce capsules and have enhanced virulence if injected into mice.

    Biofilm Streptococci have the ability to form biofilms: Densely packed bacterial communities Grow on surfaces or human tissues Bacteria secrete and surround themselves with a slimy matrix composed of polymers polysaccharides in streptococcal species. Examples of biofilms: Bacterial colonization of chronic wounds Significance: More resistant to host immune defenses More resistant to antibiotics Gram-positive bacteria: Most bacteria can be classified according to a lab procedure called Gram staining.

    Bacteria with cell walls that have a thick layer of peptidoglycan retain the crystal violet stain utilized in Gram staining but are not affected by the safranin counterstain. These bacteria appear as purple-blue on the stain, indicating that they are gram positive. The bacteria can be further classified according to morphology branching filaments, bacilli, and cocci in clusters or chains and their ability to grow in the presence of oxygen aerobic versus anaerobic.

    The cocci can also be further identified. Staphylococci can be narrowed down on the basis of the presence of the enzyme coagulase and on their sensitivity to the antibiotic novobiocin. Image by Lecturio. Now mostly of historic interest, as many more species have been described Some streptococcal species are still traditionally referred to by their serologic types groups A, B, and D. Group A: S.

    In addition, one strain E was intermediate resistant to linezolid, and one isolate E was resistant to tetracycline. Conclusions E. Different levels of antimicrobial susceptibility were observed in the tested E. This phenomenon is a cause for concern for clinicians in the treatment of dental infections caused by E. Introduction Enterococci are Gram-positive, coccus-shaped bacteria and are common colonizers of different animal hosts, plants, soil, food, and water [1,2].

    Previously, these bacteria were considered as normal commensals of the gastrointestinal tract, oral cavity, and vagina in humans [3,4]. However, there was recently an increase in nosocomial infections caused by enterococcal species, including urinary tract, bloodstream, endocardium, abdomen, biliary tract, burn wound, and endodontic infections, which were largely attributed to the antimicrobial resistance profiles [5,6,7]. Therefore, Enterococci now rank among the top three nosocomial bacterial human pathogens, and several multidrug-resistant strains have emerged that pose great therapeutic challenges [8,9].

    One of those important infection sites is the oral cavity, particularly in endodontic infections [12, 13, 14]. It has been reported as the species that is most commonly recovered from teeth with failed endodontic treatment and persistent infections, particularly in individuals with periodontal diseases [4, 9, 15].

    However, data obtained for the oral prevalence of E. In addition, the status of the oral cavity influences, directly or indirectly, the colonization by E. Genomic fingerprints are increasingly used to study relationships at the intra- or even the inter-specific level. Differences in these fingerprints between individuals are interpreted as genetic distances [17].

    However, the applied methods should provide the appropriate level of discriminatory power and be relatively efficient and cost effective. Unfortunately, there are insufficient data concerning opengl pyqt5 prevalence of E.

    Materials and methods 2. Patient selection In the present study, clinical dental root canal samples were collected from patients who were admitted to the College of Dentistry Clinics, King Saud University Riyadh, Saudi Arabia. The clinical dental specimens were collected during the period from June to May Eighty-two samples were collected from root canals, including 56 necrotic, 21 retreated, and five vital teeth.

    None of the patients had received antibiotic therapy during the preceding three months. The patients consisted of 67 males and 15 females and ranged in age from 16 to 72 years. Clinical and sampling procedures Endodontic samples were obtained from root canals according to a previously reported approach [14,21,22]. A K-type file 15 with the handle cut off was initially used for the sample collection.

    A sterile file 15 was used to agitate the canal contents for s. The file was introduced to a level about 1 mm short of the tooth apex based on diagnostic radiographs, and a gentle filing motion was applied. If the root canal was dry, a small amount of sterile saline solution was introduced into the canal to ensure viable sample acquisition.

    Murray, BE. J Infect Chemother. J Hosp Infect. Int J Antimicrob Agents. Microb Drug Resist. Performance standard for antimicrobial susceptibility testing. Expert Rev Anti Infect Ther. Performance standards for antimicrobial testing: Twentieth informational supplement.

    J Biol Chem. J Clin Invest. J Chemother. Leclercq, R, Courvalin, P. Navarro, F, Courvalin, P. Arthur, M, Courvalin, P. MS16 Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically: Approved Standard. Reynolds, PE. Euro Surveill. Mol Microbiol.

    J Infect. Pearman, JW. Clin Transplant.

    Gram positive bacteria – Enterococcus

    Armeanu, E, Bonten, MJ. Biol Blood Marrow Transplant. Transpl Infect Dis. Inflamm Bowel Dis. Am J Infect Control. Expert Opin Pharmacother. Farr, BM. Pediatr Clin North Am. Johnston, BL, Bryce, E. Tacconelli, E, Cataldo, MA. Zirakzadeh, A, Patel, R.

    Mayo Clin Proc. Hachem, R, Raad, I. Med J Aust. Can J Infect Control.

    Streptococcus

    Tanner, BD. Clin Ther. Lai, KK. Watanakunakorn, C, Patel, R. Journal of the American College of Cardiology. Tice, AD. Eur Urol. Allerberger, F, Klare, I.

    Homer-Vanniasinkam, S. Int J Surg. Clin Neurol Neurosurg. Centers for Disease Control. All rights reserved.


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